FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11054864
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA153120.13850916664375065No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG142880.12924627566653016No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA133460.12072513963084484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117150.045.158781
GGTATCA45050.041.607011
ATCAACG174300.030.3155573
TCAACGC181150.029.2070224
CAACGCA182300.029.0004445
TATCAAC192300.027.5857722
AACGCAG196700.026.9645566
TCTAGCG19500.026.88324728
ATTAATC40050.026.78668845
CTAGCGG20100.026.25341629
CGCAATA20000.026.22326336
ACGAAAG39750.025.9417356
AAAGTCG41100.025.76115659
AGGTTCG40950.025.6861567
GAACGAA41200.024.94468754
AGTCGGA43400.024.39582461
AATACGA21850.024.31914739
AGAACGA43300.023.97340653
AATCCGG23800.023.47485519
GAATCCG24250.022.6126918
Produced by FastQC (version 0.11.5)