FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9885184
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138490.14009855557569792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63600.048.7518271
GTATCAA169500.043.5226361
TATCAAC235550.031.306772
ATCAACG235300.031.1781563
TCAACGC236850.030.9731834
CAACGCA242000.030.354035
AACGCAG252900.029.1133656
ACGCAGA297600.024.7289057
CGCAGAG309250.023.8084748
AGAGTAC328750.022.39626111
GCAGAGT336050.021.971329
GAGTACT138200.021.58475312
CAGAGTA344150.021.46421810
AATCCGG26750.021.40068419
GAATCCG27400.021.39645218
AGTACAT217800.020.09290513
AGTACTT150050.020.08697113
CGGATCC28950.019.89994440
CTAGGAC31150.019.3769263
GTACTTT156400.019.31561714
Produced by FastQC (version 0.11.5)