FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11497943
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG163050.14180797382627483No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA162040.14092955583446537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103100.044.1037061
GGTATCA40850.039.2160451
ATCAACG155450.029.2013913
TCAACGC159150.028.6309954
CAACGCA161900.028.102555
ATTAATC35700.027.3441345
AAAGTCG36850.026.30277459
AATACGA16750.026.15221239
ACGAAAG36200.026.01255456
TCTAGCG16550.025.84402528
AACGCAG180700.025.1209536
CGCAATA16800.025.04815136
AGGTTCG39500.024.97423667
CTAGCGG17650.024.03791829
AGTCGGA41250.023.99865361
GAACGAA40300.023.62298854
CAATACG18550.023.61473538
TATCAAC196150.023.3717942
CTAGGAC52150.023.3484883
AGAACGA42750.022.7532753
Produced by FastQC (version 0.11.5)