FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11197087
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA155900.13923264148970174No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG135950.12141550744403432No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA112260.10025821894569543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119550.043.3224641
GGTATCA49300.041.4838941
ATCAACG174350.029.973513
TCAACGC179050.029.2441234
CAACGCA182150.028.8033585
TATCAAC194650.027.0970122
AACGCAG194500.026.9217326
TCTAGCG17500.025.23031828
AAAGTCG39250.025.22256359
CGCAATA17700.024.55502336
AGTCGGA40900.023.95179761
GAACGAA41150.023.72256354
ACGAAAG40700.023.64584456
AGGTTCG41100.023.58272767
CTAGCGG19250.023.2950429
ATTAATC42950.023.21086545
AGAACGA44700.022.0700653
AATACGA19500.021.93472139
AAGTCGG45250.021.8017160
ACGCAGA239450.021.7959087
Produced by FastQC (version 0.11.5)