FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10329087
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA149450.1446884898926691No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG138780.13435843845637083No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA111150.10760873637718416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA124100.043.449471
GGTATCA50350.040.9591941
ATCAACG187450.028.8331323
TCTAGCG17300.028.10764728
TCAACGC192550.028.104724
CAACGCA196000.027.5881465
CTAGCGG17550.027.51314529
TATCAAC202450.026.7663882
CGCAATA18300.026.21041136
AATACGA18450.026.18650839
ATTAATC33200.025.66397345
AACGCAG211400.025.6269826
AGGTTCG34050.025.21909767
ACGAAAG35350.024.58504556
AAAGTCG35750.024.21396859
GAACGAA36650.023.52502654
ATACGAA20700.023.16445540
AGTCGGA37050.022.99201661
AGAACGA37800.022.99145953
CCGCTTA5850.022.40204256
Produced by FastQC (version 0.11.5)