FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12833467
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105600.041.9041371
GGTATCA50800.031.569731
ATCAACG157800.028.0248053
TATCAAC161400.027.5084022
TCAACGC161100.027.4511684
CAACGCA164600.027.1401145
AACGCAG177150.025.2369736
CTAGGAC58600.022.4867843
CGCGAAT9550.021.31164728
AAAGTCG26850.020.9412859
CTCTAGG64400.020.3652211
TCTAGCG14000.019.95838228
ACGCAGA222250.019.914077
ATTAATC29500.019.17772145
ACGAAAG29350.019.15759856
GGTCTAG29650.019.0074561
AATACGA14050.018.90489839
CGCAGAG236250.018.7924588
CTAGCGG14950.018.6900529
GAACGAA30450.018.57874754
Produced by FastQC (version 0.11.5)