FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10157217
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131150.12912001387781713No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA114610.11283602585235701No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG112520.11077837561213864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64300.049.1871531
GTATCAA171550.043.989971
ATCAACG245500.030.7402443
TCAACGC249800.030.1960894
TATCAAC251450.030.0958862
CAACGCA253100.029.8123425
AACGCAG266800.028.3330616
TCTAGCG13100.025.01005228
CGCAATA13200.024.83451736
ACGAAAG25900.023.56859256
ACGCAGA321750.023.547687
AAAGTCG26450.023.33939659
CTAGCGG14000.023.15649229
GAATCCG24350.022.80184218
CGCAGAG334300.022.6531358
GAACGAA27350.022.57159654
AGGTTCG27700.022.16068667
AGAGTAC347150.021.80489511
GAGTACT134300.021.74902312
AGTCGGA29250.021.5766161
Produced by FastQC (version 0.11.5)