FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10157217
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA116350.042.633831
GGTATCA46450.042.4609261
ATCAACG166050.029.9338043
TCAACGC172100.029.0021934
CAACGCA175050.028.5933885
TATCAAC179000.028.0019232
AACGCAG185400.026.8481756
AAAGTCG26250.022.9955359
TCTAGCG14100.022.26239628
CGCAATA14000.022.17502236
CTAGCGG14600.021.97251129
ACGCAGA225950.021.9230167
ACGAAAG28300.021.45197756
AGTCGGA27850.021.30286261
CGCAGAG236750.020.9084688
AGGTTCG27800.020.84466467
GAACGAA29900.020.53446854
ATTAATC30700.020.33733745
AGAACGA30400.020.31049553
GAATCCG25450.020.194718
Produced by FastQC (version 0.11.5)