FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10446839
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236390.22627897299843525No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA145310.13909470606371938No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG144370.13819491235578532No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG130310.12473629583072927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109050.10438564239383798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81800.050.0105971
GTATCAA245400.046.859091
ATCAACG346200.033.2551573
TATCAAC349650.032.9575652
TCAACGC352800.032.6910674
CAACGCA361950.031.8423865
AACGCAG380900.030.2751566
ACGCAGA448450.025.730567
GAGTACT204100.025.496812
CGCAGAG462550.024.9608848
AGAGTAC469500.024.54790511
GTACTTT217500.023.95755814
CAGAGTA485950.023.8087310
TCTAGCG16700.023.74845328
GCAGAGT487800.023.7395299
CTAGCGG17050.023.6669629
TACTTTT224650.023.25656715
AGTACTT227800.022.98039413
ACTTTTT231000.022.7964116
AAAGTCG29800.022.68319159
Produced by FastQC (version 0.11.5)