FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10625261
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA138950.044.134181
GGTATCA55050.042.3616141
ATCAACG199400.030.7902433
TATCAAC204950.030.0411622
TCAACGC204100.029.9962844
CAACGCA206750.029.6245885
AACGCAG220300.027.8174696
GAATCCG20800.023.37762618
CTAGCGG9750.022.99530829
AATCCGG21000.022.66232519
ACGCAGA271950.022.5342457
TCTAGCG10200.021.97770728
CGCAGAG283200.021.6269058
CGAAATT12650.021.5389348
AGAGTAC293500.020.86852611
AAAGTCG22500.020.84633359
CGCGAAT3850.020.60327728
CTAGGAC42800.020.227783
GCAGAGT308700.019.9298929
CAGAGTA308550.019.90614310
Produced by FastQC (version 0.11.5)