FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10625261
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95800.041.1568871
GGTATCA44400.035.44241
ATCAACG135650.029.040053
TCAACGC138700.028.6254234
CAACGCA142950.027.7748955
TATCAAC142750.027.7420962
AACGCAG153850.025.784676
CTAGTAC14100.020.7946113
TACCGGT11900.020.2914167
TATATCG11450.020.18432460
ACGCAGA198650.019.9870197
AAAGTCG23500.019.22844959
TCTAGCG11400.019.0626228
CGCAGAG209150.019.0165928
AATACGA11700.018.86839139
CTAGGAC43500.018.7143083
CTAGCGG12000.018.68422129
GAACGAA25000.018.6267354
CGCAATA12000.018.3967736
ACGAAAG24800.018.35985456
Produced by FastQC (version 0.11.5)