FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10620060
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA122900.1157243932708478No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG120540.11350218360348246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133850.042.2922741
GGTATCA52700.041.3853721
TCTAGCG12000.029.31448728
ATCAACG192550.029.2326813
TCAACGC196950.028.6487054
CAACGCA199350.028.2842355
TATCAAC202400.028.0151842
CTAGCGG13050.027.48698229
AATACGA12800.027.22725339
CGCAATA13000.026.80786936
AACGCAG212550.026.575876
AAAGTCG26000.025.99873759
ACGAAAG26900.023.8461356
CAATACG15400.023.07846538
ATTAATC28750.023.0381445
ATACGAA15300.022.99771140
GAACGAA28700.022.47082354
CGCGTTA1103.2800774E-521.94595114
AGGTTCG29750.021.7928467
ACGCAGA260250.021.7314077
Produced by FastQC (version 0.11.5)