FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10620060
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA106680.10045140987904022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98400.040.1957551
GGTATCA43050.035.54921
ATCAACG141150.028.1032353
TCAACGC143100.027.7678344
CAACGCA145350.027.0297455
TATCAAC152700.026.2503492
AACGCAG156200.025.2846956
AAAGTCG27500.024.58446159
ACGAAAG28400.023.6840356
ATTAATC29450.023.54280945
AGGTTCG28650.023.11574267
GAACGAA29550.022.6455954
TCTAGCG13400.021.87989828
AGAACGA31800.021.58565953
AGTCGGA32050.020.98668961
CTAGCGG14100.020.79356629
ACGCAGA193550.020.280867
GAATCCG22800.019.97011218
GGTCTAG22300.019.585471
AATACGA14750.019.40990439
Produced by FastQC (version 0.11.5)