FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10556244
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA106160.10056607255383637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA142400.044.8234251
GGTATCA53650.043.890111
ATCAACG201500.031.4961663
TCAACGC203200.031.3001174
TATCAAC206300.030.863952
CAACGCA207350.030.6713375
AACGCAG218700.029.1101446
ACGCAGA266500.023.9279377
AATCCGG25700.023.61855719
GAATCCG27050.022.82230618
CGCAGAG281100.022.6605038
TCTAGCG11450.022.59122828
CGCAATA11250.022.39084436
CTAGCGG11850.021.83010329
AGAGTAC293200.021.7138811
CAGAGTA302350.021.15931310
GCAGAGT309150.020.7048559
CGCGAAT3200.020.47800628
ACGAAAG27050.020.40069856
AGTACAT219600.020.33765813
Produced by FastQC (version 0.11.5)