FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10556244
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99300.043.4901051
GGTATCA42150.037.1593251
ATCAACG142600.030.1888353
TCAACGC145450.029.6680324
CAACGCA148250.029.085115
TATCAAC152300.028.3801572
AACGCAG157850.027.2723986
ACGCAGA196400.021.7612157
AATCCGG26700.021.446719
GAATCCG26850.021.1982118
TCTAGCG11650.020.72608428
CGCAGAG210600.020.2284188
ATTAATC26600.020.10081745
AAAGTCG26600.019.97018859
AGGTTCG26400.019.72903467
AGAGTAC213100.019.69955311
AGTACAT210850.019.66390613
ACGAAAG27550.019.40676556
TAACCGC7300.019.37359653
CTAGCGG13250.019.0043129
Produced by FastQC (version 0.11.5)