FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10035432
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA132340.13187274847759417No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG129970.129511116213034No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA100750.10039428297655748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135050.044.2246741
GGTATCA53200.043.1437531
TCAACGC196350.030.6008074
ATCAACG196700.030.4603523
CAACGCA198850.030.0728245
TATCAAC205200.029.3678592
AACGCAG211450.028.2962936
AAAGTCG33150.024.23995459
ACGAAAG32850.023.9366456
ATTAATC34250.023.66966845
GAACGAA33850.023.63679954
AGGTTCG35150.023.44917967
ACGCAGA260850.022.9377467
TCTAGCG12650.022.90123728
AGTCGGA36300.022.23138261
CGCAGAG270150.022.160768
AGAGTAC276500.021.63935311
CCGCTTA6300.021.34950356
AGAACGA38250.021.27802353
CTAGCGG13500.021.2055329
Produced by FastQC (version 0.11.5)