FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9774264
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152360.1558787444251557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69900.048.7789041
GTATCAA180950.043.0532261
TATCAAC257250.030.5306952
ATCAACG255950.030.509793
TCAACGC260350.030.0332954
CAACGCA265900.029.3890935
AACGCAG276950.028.2286646
ACGCAGA327700.023.8990617
CGCAGAG336400.023.2704938
GAGTACT148850.023.09876412
AGAGTAC346050.022.5421911
GCAGAGT365400.021.5274439
CAGAGTA371000.021.15613210
AGTACTT166600.020.69996513
GTACTTT167400.020.6425614
TACTTTT174650.019.98311615
ACTTTTT175650.019.81044616
GAATCCG20700.019.32566618
CGTCGAG19200.019.21903617
TTCGTCG19300.019.11955515
Produced by FastQC (version 0.11.5)