FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10329424
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA112250.041.622441
GGTATCA48350.039.6195221
ATCAACG156900.029.3279953
TCAACGC160750.028.6466274
TATCAAC161900.028.5737462
CAACGCA164550.028.2374525
AACGCAG173600.026.6264326
ACGCAGA215200.021.3992737
CGCAGAG227750.020.1292048
AGAGTAC230350.019.8869311
CTAGGAC54550.018.9703773
AGTACAT234200.018.8818413
CAGAGTA245350.018.83990710
GCAGAGT253050.018.198569
GAGTACA250150.017.7885612
TACCGGT12500.017.6615987
AAAGTCG20300.016.99177459
CTCTAGG59350.016.8142951
GTACATG317200.016.6872861
CACGTAA15450.016.29796465
Produced by FastQC (version 0.11.5)