FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10075874
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA125650.12470382221929334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124120.12318534352454188No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG122980.12205392802649179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68000.048.9252551
GTATCAA181400.044.436081
ATCAACG260550.031.018253
TCAACGC264700.030.6093714
CAACGCA270750.029.8581375
TATCAAC276150.029.3550342
AACGCAG287200.028.219446
ACGCAGA338650.023.9321527
AAAGTCG26100.023.91905259
CGCAGAG350150.023.1460328
AGAGTAC363100.022.31102611
TCTAGCG16150.022.20949728
ACGAAAG27850.022.16814656
AGGTTCG27450.022.11280867
GAACGAA28400.021.73883454
CAGAGTA375550.021.64485210
GAGTACT135850.021.62952812
AATACGA16300.021.59447739
TACCGGT14400.021.5548487
ACCGTCG25350.021.3591758
Produced by FastQC (version 0.11.5)