FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10075874
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA108850.10803033066908141No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG104580.10379248490006922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127050.042.290521
GGTATCA50900.042.1281471
ATCAACG177350.030.3615323
TCAACGC181050.029.8927334
CAACGCA184900.029.401625
AACGCAG197600.027.3894736
TATCAAC195750.027.386042
TCTAGCG14450.024.58801728
CGCAATA14100.024.4640636
CTAGCGG15450.022.99644729
ACGCAGA236000.022.7574867
AATACGA15600.022.33297739
CGCAGAG243700.022.0103458
TCGGATA6300.021.35384448
AGAGTAC249350.021.30389611
AAAGTCG26500.020.56622559
AGTACAT251500.020.50458113
ATAACCG6650.020.2304652
ACGAAAG28500.020.09163356
GCAGAGT265850.020.0338699
Produced by FastQC (version 0.11.5)