FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10406273
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61950.044.8310551
GTATCAA167500.044.549161
ATCAACG238550.031.3310593
TATCAAC241750.031.1032
TCAACGC241300.030.9446624
CAACGCA245100.030.4489255
AACGCAG259150.028.8774096
ACGCAGA312300.023.9958067
ATAACCG7250.023.7845152
TAACCGC7150.023.15269553
CGCAGAG325050.023.0545758
GAGTACT126050.022.68106512
CGCGAAT7000.022.66378228
AATCCGG23600.022.50425519
AGAGTAC335900.022.32036411
GAATCCG24550.021.91437318
CCGCTTA7400.021.905356
GCAGAGT350300.021.4421049
TCGGATA7950.021.25810648
CAGAGTA358150.021.01064910
Produced by FastQC (version 0.11.5)