FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11024649
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA141570.12841225149208832No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG135260.12268871326424995No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA116210.10540925157798675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109850.043.472761
GGTATCA44850.042.4658051
ATCAACG160050.029.7384453
TCAACGC164300.029.010784
CAACGCA166900.028.5966745
TATCAAC171050.028.0092562
ATTAATC32150.027.79024945
ACGAAAG31450.027.41452656
GAACGAA32050.026.90118254
AACGCAG180850.026.3907286
AGGTTCG33300.026.20183867
AAAGTCG33850.025.7766959
AATCCGG23100.024.03650719
AGAACGA35650.023.9908553
AGTCGGA37650.023.81615861
TATATCG20200.023.56085460
TAACGGC6400.022.64514236
AATACGA14250.022.2776239
GAATCCG24500.022.09983318
CGCAATA14700.022.06562236
Produced by FastQC (version 0.11.5)