FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11024649
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA124530.11295597710185602No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG117100.10621653351503527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87650.042.3666761
GGTATCA38500.035.024311
CGCAATA14700.029.56618136
ATCAACG127350.029.3880563
TCAACGC130400.028.9115144
CAACGCA134200.028.2225235
AATACGA16350.027.21498339
TATCAAC141450.026.4364912
TCTAGCG16500.026.132228
ACGAAAG29100.025.84065856
AACGCAG146400.025.7292736
CAATACG17700.025.52948438
CTAGCGG16950.025.43819429
ATTAATC30800.024.75047745
GAACGAA31000.024.03433654
ATACGAA19150.023.23566240
AAAGTCG32650.022.81963359
CTAGGAC36000.022.7083663
AGGTTCG32550.022.67687267
AGAACGA33500.022.13766353
Produced by FastQC (version 0.11.5)