FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10509863
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA147580.14042047931547727No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG138920.13218060026091683No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA118910.1131413416140629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94150.043.9255371
GGTATCA42450.034.5639881
ATTAATC32000.029.6457745
ATCAACG139650.029.5888233
CGCAATA12900.028.88881936
TCAACGC144450.028.676284
ACGAAAG32550.028.28837656
CAACGCA146350.028.2767895
TCTAGCG12750.027.58911128
TATCAAC151700.027.3754332
AGGTTCG34300.027.24713767
AAAGTCG34700.027.23145759
AATACGA14050.026.52526539
AACGCAG157400.026.3789276
GAACGAA35300.026.27987354
CTAGCGG13350.026.09430329
AGAACGA36350.025.4256453
ATACGAA15500.023.81330540
AGTCGGA39750.023.68510661
GCGCAAT15600.023.66808935
Produced by FastQC (version 0.11.5)