FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10457281
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA104880.10029375704831878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135450.043.5679051
GGTATCA59700.040.8989031
ATCAACG194950.030.1510773
TCAACGC199000.029.5195524
CAACGCA202650.028.9820465
TATCAAC206700.028.6382052
AACGCAG211250.027.802056
ATTAATC27750.022.99602545
ACGCAGA260300.022.5899547
ACGAAAG27600.022.3673956
AGGTTCG28450.022.18380467
AAAGTCG29650.021.6355559
CGCAGAG272850.021.5378578
TCTAGCG12600.021.34987828
AGTCGGA29950.021.18842161
CGCAATA13100.020.80801836
AGAACGA29500.020.69256453
GAACGAA29200.020.66913854
CTAGCGG13350.020.41015229
CGTCGTA19650.020.35880310
Produced by FastQC (version 0.11.5)