FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10929457
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA202310.18510526186250606No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG183270.16768445129524734No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG147490.13494723479858148No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCG139940.12803929783519893No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG133260.12192737480004724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA154100.046.501291
GGTATCA56100.044.2178341
ATCAACG232900.030.8797263
TCAACGC238600.030.1999954
CAACGCA245100.029.4131725
TATCAAC246550.029.2838972
AACGCAG264050.027.276166
GTACTAG7800.023.7401391
ATTAATC26350.022.90942445
ACCGTCG43300.022.3851138
ATACCGT44000.022.3424636
ACGCAGA328050.021.9338637
CGTCGTA40200.021.88068610
CTAGGAC52900.021.8436893
TACCGTC47400.021.6131957
TATATCG18850.021.59243260
CGCAATA16350.021.51915736
CGCAGAG345800.020.788048
CAGAGTA347400.020.64293710
AGAGTAC347700.020.60509511
Produced by FastQC (version 0.11.5)