FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9793773
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106450.043.226851
GGTATCA45200.040.132411
ATCAACG151150.030.3255633
TCAACGC152100.030.1807334
TATCAAC152800.030.180212
CAACGCA154950.029.5780835
AACGCAG164000.027.9241356
AATCCGG23550.023.2829719
GAATCCG23850.022.55632818
ACGCAGA207150.022.0741567
CGACTAT7450.021.29795531
ATTAATC16900.021.22878645
CGCAGAG218550.020.9226178
CTAGGAC33800.020.2041453
TATCGTC20350.020.1665963
ACGAAAG17500.020.10135756
CGAAATT8450.019.99985948
AGAGTAC229900.019.85998711
CCGCTTA6550.019.48155456
CAGAGTA237400.019.33405110
Produced by FastQC (version 0.11.5)