FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10773871
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA152380.14143477307274238No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG145400.1349561360071974No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA110950.10298062785418537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122350.044.091141
GGTATCA48400.040.8846661
ATCAACG177400.030.3715023
TCAACGC180600.029.9471194
CAACGCA183500.029.474255
TATCAAC194450.027.6926172
AACGCAG195250.027.6129516
AAAGTCG41550.025.56892659
ATTAATC42600.024.29196245
AGTCGGA43250.024.16502661
ACGAAAG42850.024.06850656
AGGTTCG43500.023.62901967
GAACGAA43650.023.54847554
CTAGCGG19650.023.52258929
CGCAATA19950.023.16832736
AATACGA19950.023.16811439
TCTAGCG20200.022.88212228
ACGCAGA238250.022.528057
AGAACGA46500.022.25353853
AATCCGG22750.021.37826719
Produced by FastQC (version 0.11.5)