FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10430107
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218440.2094321755280171No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG125190.12002753183644234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83750.049.3465461
GTATCAA240900.046.314191
TATCAAC329600.033.9072462
ATCAACG340050.032.8335653
TCAACGC341150.032.686474
CAACGCA348950.031.9708525
AACGCAG366450.030.4059896
ACGCAGA428700.026.0070557
CGCAGAG442050.025.2294378
AGAGTAC450200.024.75786211
GAGTACT202450.024.222612
CAGAGTA468750.023.9100810
GCAGAGT478650.023.4155459
GTACTTT216550.022.75700414
TACTTTT227000.021.80042615
AGTACTT227750.021.60760513
ACTTTTT230000.021.56115716
AGTACAT296850.021.01563613
GAGTACA308000.020.28833612
CTAGGAC59950.019.7343583
Produced by FastQC (version 0.11.5)