FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10339136
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156620.15148267708249508No Hit
GTATGTGGGAAGCCCAAGAATCCGGCAAACCCAGTGCAGCGGATCCTGGGTGGACACCTGGATGCCAAAGGCAGC121980.11797891042346284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62100.048.678761
GTATCAA174300.045.405931
TATCAAC237500.033.374222
ATCAACG240900.032.8023223
TCAACGC244050.032.420554
CAACGCA247850.031.9196265
AACGCAG260700.030.3720176
ACGCAGA310000.025.5863847
CGCAGAG325050.024.4122128
GAATCCG37600.024.03083418
AGAGTAC338250.023.36823311
GAGTACT151700.023.23337412
AATCCGG39850.022.93352519
CAGAGTA352050.022.5889310
GCAGAGT356250.022.3032579
TATATCG22100.021.53611660
TACTTTT165600.021.47138415
GTACTTT164850.021.40139414
CTAGGAC38000.021.2397083
AGTACTT166000.021.19049813
Produced by FastQC (version 0.11.5)