FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10607683
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112950.1064794262799897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59100.045.060451
GTATCAA158200.044.992781
ATCAACG220850.032.2202223
TATCAAC223000.032.003142
TCAACGC223800.031.7937154
CAACGCA227700.031.214745
AACGCAG240750.029.5508216
ACGCAGA289950.024.5721867
CGCAGAG307650.023.1918878
AGAGTAC315700.022.59034211
GAGTACT133050.022.5500312
GAATCCG22250.022.47348818
CTAGGAC42150.022.1767753
CGCAATA9650.021.81486136
TATATCG16750.021.41401760
CAGAGTA335350.021.35869810
GCAGAGT336550.021.313189
TCTAGCG10300.020.7590528
AATCCGG24650.020.425319
CACGCGA5100.020.2858813
Produced by FastQC (version 0.11.5)