FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10607683
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98750.041.929761
GGTATCA43100.035.641021
ATCAACG139550.029.2172493
TCAACGC143100.028.7089734
CAACGCA143750.028.6755495
TATCAAC143200.028.6668642
AACGCAG152700.027.0170366
CTAGGAC39600.021.428493
ACGCAGA192550.021.3360397
TATATCG15850.021.10949160
TACCGGT17200.020.255297
CGCAGAG203900.020.0808058
AGAGTAC210500.019.27066211
AATCCGG24100.019.03601519
AGTACAT207350.018.8975513
GAATCCG24400.018.66059718
TTCGTCG18200.018.57316615
CAGAGTA227850.017.90940910
CTCTAGG51000.017.8667321
GCAGAGT228750.017.7787139
Produced by FastQC (version 0.11.5)