FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10375133
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA105550.10173363560737005No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG103930.1001722098405871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59050.044.5845871
GTATCAA153900.044.209331
ATCAACG214100.031.8667033
TCAACGC218300.031.2371964
TATCAAC223850.030.6333962
CAACGCA222650.030.6230645
AACGCAG234150.029.1333566
ACGCAGA286600.023.7535977
AATCCGG20900.023.26684819
CGCAGAG302050.022.5383728
AAAGTCG29450.021.78277859
AGAGTAC315650.021.58955811
TCTAGCG12800.021.28529728
ACGAAAG29750.021.21491856
GCAGAGT325550.021.017369
CAGAGTA325750.021.01504510
ATTAATC31050.020.77489945
CGCAATA13000.020.70331836
CTAGCGG13200.020.64178329
AGTACAT243200.020.53352213
Produced by FastQC (version 0.11.5)