FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10375133
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117850.041.639131
GGTATCA49200.041.4694141
ATCAACG162950.030.1858773
TCAACGC165800.029.7715974
CAACGCA170350.028.9766855
TATCAAC177500.027.7330552
AACGCAG182500.027.0857476
ACGCAGA222550.022.0564087
CGAAATT10450.021.12598648
CGCAGAG237950.020.585448
AATCCGG20350.020.34119219
AGTACAT238900.019.76623213
TCTAGCG13100.019.74901228
AGAGTAC247000.019.53716511
CTAGCGG13750.019.31697829
CGCAATA13350.019.12042236
GCAGAGT256350.018.9597689
ACCGTCG26400.018.9461778
AAAGTCG28200.018.8375759
AATACGA14300.018.81487839
Produced by FastQC (version 0.11.5)