FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10555272
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA124930.11835791630949918No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG117170.11100613987019947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105550.043.4765741
GGTATCA44850.037.071661
ATCAACG158250.028.9013353
TCAACGC160600.028.4773564
CAACGCA163050.028.045735
TATCAAC169650.027.1431272
AACGCAG174550.026.2368686
ATTAATC28700.025.7215645
ACGAAAG29050.024.69263356
AGGTTCG28900.024.58201667
AAAGTCG30800.023.96191259
GAACGAA30350.023.63529654
TCTAGCG13000.023.61015928
CGCAATA13300.023.35105736
CTAGCGG13450.022.82379529
AGTCGGA33100.022.29688661
AGAACGA32500.022.17742253
AATACGA14000.021.9382739
AAGTCGG35100.021.3211760
GAATCCG23100.021.04945818
Produced by FastQC (version 0.11.5)