FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10555272
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA111100.10525545907296373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88450.041.144351
GGTATCA41450.032.8609661
ATCAACG132900.028.3167173
TCAACGC136100.027.7517834
CAACGCA139200.027.3326325
TATCAAC141550.026.0276762
AACGCAG148400.025.4986936
AGGTTCG27500.024.2076967
ATTAATC28450.024.00691645
TCTAGCG14250.023.48010328
ACGAAAG29150.022.83809356
GAACGAA29950.022.6885354
CGCAATA14750.022.45041836
AAAGTCG31050.021.66267259
CTAGCGG15150.021.62977829
AGTCGGA31850.021.22645461
TACCGGT9300.021.1416657
CTAGGAC34500.021.096313
AGAACGA32950.021.04174253
AATACGA15950.020.97722439
Produced by FastQC (version 0.11.5)