FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9544106
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43750.040.515181
GTATCAA104950.040.3616031
ATCAACG140900.030.0139263
TCAACGC145100.029.1454644
TATCAAC145850.028.9736082
CAACGCA148650.028.5658975
AACGCAG155000.027.3511076
ACGCAGA186600.022.5344417
CGCAGAG196100.021.4603588
AGTACAT197800.020.47242213
AGAGTAC205600.020.33346711
GCAGAGT214750.019.4840879
CAGAGTA218000.019.36726410
AATCCGG19150.019.27370319
TTCGTCG17900.018.8843815
GAGTACA217600.018.78414512
GAATCCG20800.017.74468618
TGTTTCG19400.017.4243412
CTAGGAC34100.017.297573
TAACCGC7450.017.1316953
Produced by FastQC (version 0.11.5)