FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10630314
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA112600.043.0338481
GGTATCA50800.037.2590981
ATCAACG166100.029.0310753
TCAACGC168550.028.5670834
CAACGCA169600.028.4061495
TATCAAC172950.027.9416052
AAAGTCG28050.027.90996759
ATTAATC28300.027.18272645
ACGAAAG28100.027.0006756
AACGCAG181150.026.6136536
GAACGAA29100.025.95380854
AGGTTCG31700.024.04279967
CGCAATA12200.024.04256836
AGTCGGA32950.023.96869361
TCTAGCG12250.023.3665228
TACCGGT13050.022.9903987
AGAACGA33400.022.92199353
CTAGCGG13150.022.81781829
CGCGAAT7000.022.6627128
CTAGGAC36650.022.493113
Produced by FastQC (version 0.11.5)