FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10361465
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA138790.13394823994483407No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG132080.12747232172284517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95700.042.558561
GGTATCA41050.038.0686381
ATCAACG142350.028.6897473
TCAACGC146200.028.1460294
ATTAATC30800.027.7740845
CAACGCA150750.027.365425
TATCAAC155500.026.4207572
ACGAAAG32450.026.3613356
AAAGTCG32500.026.10813359
GAACGAA34150.025.55432354
AACGCAG161250.025.4126056
AGGTTCG33200.025.3496367
AGTCGGA35050.024.60208361
TATATCG14350.023.79595660
AGAACGA37050.023.64721753
TCTAGCG15050.023.14887628
GAATCCG24200.022.5204118
CGCAATA15350.022.47141336
AATACGA15150.022.0844939
TACCGGT16400.021.8738967
Produced by FastQC (version 0.11.5)