FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9596568
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94200.041.2954521
GGTATCA41750.038.3068961
ATCAACG130150.029.869263
TCAACGC133300.029.1370894
CAACGCA134800.028.7365475
TATCAAC138100.028.1760752
AACGCAG143700.027.0293356
TATATCG16400.024.1879360
TACCGGT19000.021.4232047
ACGCAGA181500.021.2291497
GAATCCG21750.020.61694318
CGCAGAG191850.020.0841858
CTAGGAC34000.019.8848293
AATCCGG22400.019.71080619
AGAGTAC194800.019.69118711
AGTACAT190050.019.47455213
CAGAGTA207300.018.50401710
CGCAATA8450.018.36925136
GAGTACA205600.018.08619112
GCAGAGT212600.018.0103659
Produced by FastQC (version 0.11.5)