FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11350015
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98500.042.306091
GGTATCA43950.039.2614821
ATCAACG141650.029.659593
TATCAAC141800.029.5318262
TCAACGC144600.029.0545044
CAACGCA148250.028.3628065
AACGCAG157050.026.6198046
GAATCCG33500.024.40249318
CGCGAAT6450.022.99602328
CGCAATA9700.021.6918436
TCTAGCG9900.021.60232528
ACGCAGA196150.021.1201427
TATATCG19850.020.678560
AATCCGG38950.020.63397419
CTAGGAC43800.020.5542133
TACCGGT20900.020.300237
TATCGTC23100.020.00908763
CGCAGAG207550.019.9104028
AAAGTCG17350.019.68230259
AATACGA11000.019.44175139
Produced by FastQC (version 0.11.5)