FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10601744
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112920.10651077784938025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA162100.043.760241
GGTATCA65400.040.6471941
ATCAACG223650.031.9390543
TCAACGC225350.031.712074
CAACGCA228750.031.2216725
TATCAAC230600.031.1424482
AACGCAG241700.029.605226
ACGCAGA294400.024.35257
CGCAGAG306750.023.3721588
AGAGTAC310650.023.03444311
GAGTACT126350.021.88951912
CAGAGTA329600.021.77277610
GCAGAGT333550.021.5976479
ACGAAAG23900.021.50077856
AAAGTCG25000.021.24439859
TCTAGCG12000.020.98048828
CGTCGTA17550.020.63198910
CTAGGAC41600.020.3963583
ATACCGT24550.020.3677656
GAACGAA26350.020.1558854
Produced by FastQC (version 0.11.5)