FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10601744
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102650.040.097111
GGTATCA51250.031.8112851
ATCAACG144700.028.3672223
TCAACGC145850.027.9538234
CAACGCA149000.027.6182795
TATCAAC153750.026.7443642
AACGCAG160500.025.5750476
TCTAGCG15000.023.68626828
CTAGCGG16350.022.15226429
AAAGTCG24050.021.22662259
ACGAAAG24350.020.82374256
AGGTTCG24600.020.61163367
GAACGAA25400.020.3704254
ACGCAGA200900.020.3289177
AATACGA17150.020.31448739
CGCAGAG207550.019.6611338
AGTCGGA26500.019.65446761
AGAGTAC208600.019.34692811
ATTAATC27150.019.18563745
CAATACG18650.019.05070738
Produced by FastQC (version 0.11.5)