FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10324976
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA172610.16717714404372466No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG171550.1661505072747869No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG118450.114721816302527No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG106450.1030995132579485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75700.050.804381
GTATCAA201750.044.635791
ATCAACG286650.031.454723
TATCAAC291800.030.9591272
TCAACGC292150.030.8730164
CAACGCA297650.030.3092865
AACGCAG313050.028.7959566
ACGCAGA373500.024.144647
CGCAGAG388600.023.2063278
ATAACCG6850.023.15909852
CGAAATT11750.022.60092248
AATCCGG28200.022.50144819
AGAGTAC401800.022.42689311
CAGAGTA404100.022.3418110
GCAGAGT404800.022.3031759
TCGGATA7150.021.70600148
CGCAATA12100.021.67348536
TCTAGCG12200.021.4835528
GAATCCG29600.021.4371918
AGTACAT330700.021.40900613
Produced by FastQC (version 0.11.5)