FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10824296
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110450.10203896863130867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61850.045.164321
GTATCAA158900.044.5440371
ATCAACG231000.030.3713173
TATCAAC235550.029.8882562
TCAACGC235600.029.747814
CAACGCA237850.029.448235
AACGCAG250350.028.0740456
TCTAGCG10700.023.85142928
AAAGTCG25850.023.74793859
ACGCAGA307400.022.9087987
ACGAAAG26650.022.77591956
AGAACGA27250.022.52713253
GAACGAA27550.022.03167354
CGCAGAG321850.021.8691488
CTAGCGG12000.021.8432329
AGTCGGA28750.021.83222661
AGGTTCG28550.021.3808867
AGAGTAC330500.021.2656711
AAGTCGG30700.020.67016260
CGCAATA12200.020.64734636
Produced by FastQC (version 0.11.5)