FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10824296
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105950.042.885071
GGTATCA45000.037.019941
ATCAACG152850.029.7659113
TCAACGC157600.028.9555234
CAACGCA159700.028.5323625
TATCAAC162450.028.0503942
AACGCAG169300.026.8737136
GAATCCG23650.021.29470318
AAAGTCG25750.021.16516759
ACGCAGA216750.020.9269757
AGGTTCG26700.020.54053167
ATTAATC27850.020.31311445
CGCAGAG227850.019.8924458
AGAACGA28500.019.72806753
ACGAAAG27750.019.63956656
AATCCGG24700.019.55153819
GAACGAA27800.019.48034954
CGCAATA12250.019.42946436
TACCGGT11150.019.1803557
AGAGTAC235300.018.96959911
Produced by FastQC (version 0.11.5)