FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10946393
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109750.044.6294331
GGTATCA44800.036.6520081
ATCAACG162400.030.351193
TCAACGC165850.029.9065954
CAACGCA170150.029.2731025
TATCAAC174300.028.2024382
AACGCAG180450.027.411186
AATCCGG36400.026.72287619
GAATCCG38300.026.11756718
CGGATCC43000.022.13962240
TATATCG31700.021.97999660
ATCCGGC44650.021.93968420
ACGCAGA224150.021.8209157
AGCGGAT45450.021.32631538
TATCGTC16350.021.09714563
GCGGATC45500.020.99915739
CGCAGAG233450.020.9073988
AAAGTCG31250.020.08902559
AGGTTCG31100.020.0747367
TACCGGT35300.020.0315537
Produced by FastQC (version 0.11.5)