FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11182918
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA197180.17632249471917796No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG196460.175678655606703No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA115170.1029874313663035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA175550.044.609321
GGTATCA63500.044.383921
ATCAACG254000.030.756373
TCAACGC258750.030.2310754
TATCAAC263250.029.8596252
CAACGCA263100.029.7140165
AACGCAG280950.027.8259056
TCTAGCG15550.023.50832628
AAAGTCG36550.023.40075959
ACGCAGA341750.022.8856537
GAACGAA37450.022.56222254
CTAGCGG16650.022.37167429
ACGAAAG38300.022.33183356
AAAGCGA23850.022.13712525
ATTAATC38650.022.13306845
CGCAGAG354850.022.0405858
AGTCGGA39750.021.51673361
AGAGTAC365950.021.31579611
AAGTCGG40550.021.26233560
CGCGAAT5200.021.2223228
Produced by FastQC (version 0.11.5)