FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10155053
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91300.042.5888631
GGTATCA39800.034.5847631
ATCAACG135400.029.14423
TATCAAC134950.029.0900252
TCAACGC137300.028.8402524
CAACGCA141150.028.2257775
AACGCAG150500.026.2426326
TACCGGT20700.022.3292927
AATCCGG24750.022.29899419
GAATCCG25350.021.90684918
ACGCAGA187850.020.9698547
CTAGGAC42850.020.9299093
CGCAATA8800.019.99129136
GTGTACG26400.019.849931
CGCAGAG199250.019.7009248
TGTACGT22950.019.3901772
TATATCG22300.019.17972460
AGAGTAC207300.018.98561711
AGTACAT203900.018.92965513
CAGAGTA213550.018.6562810
Produced by FastQC (version 0.11.5)