FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004978103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004978103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10342205
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139100.13449743067363293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63700.045.777491
GTATCAA165800.044.4307561
TATCAAC231250.032.0557252
ATCAACG230700.032.04123
TCAACGC234050.031.493554
CAACGCA238200.030.9712755
AACGCAG250250.029.4796666
ACGCAGA304250.024.2814567
CGCAGAG319650.023.1008428
AGAGTAC323300.022.79736511
CAGAGTA340150.021.76944710
GCAGAGT343500.021.567189
GAGTACT142400.021.53166812
GAATCCG20800.021.39069418
CTAGGAC38050.020.3061453
AGTACAT213300.020.21180213
CGCGAAT6800.019.7814628
GTACTTT157300.019.53624714
TACCGGT10650.019.4306587
AGTACTT159650.019.35641513
Produced by FastQC (version 0.11.5)